Home
HelpTest Code CALX CALR Mutation Analysis, Myeloproliferative Neoplasm (MPN), Reflex, Varies
Reporting Name
CALR, Gene Mutation, Exon 9, ReflexSpecimen Type
VariesSpecimen Required
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
Submit only 1 of the following specimens:
Specimen Type: Whole Blood
Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
3. Label specimen as blood.
Specimen Stability Information: Ambient (preferred)7 days/Refrigerate 7 days
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient (preferred)7 days/Refrigerate 7 days
Specimen Type: Extracted DNA from blood or bone marrow
Container/Tube: 1.5- to 2-mL tube
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA from blood or bone marrow.
2. Provide volume and concentration of the DNA on the label.
Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient
Specimen Minimum Volume
Whole blood/Bone marrow: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | 7 days |
Reference Values
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
An interpretive report will be provided.
Specimen Retention Time
Whole blood/Bone marrow: 2 weeks; Extracted DNA 3 monthsPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81219-CALR (calreticulin) (eg, myeloproliferative disorders), gene analysis, common variants in exon 9
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CALX | CALR, Gene Mutation, Exon 9, Reflex | 77174-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 36998 | Final Diagnosis | 22637-3 |
Method Name
Only orderable as a reflex. For more information see MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies.
Polymerase Chain Reaction (PCR) and Fragment Analysis
Secondary ID
36997Useful For
Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder
Evaluating mutations in CALR in an algorithmic process for the MPNR / Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies
Reject Due To
| Gross hemolysis | Reject |
| Paraffin shavings | Reject |
| Slides | Reject |
| Moderately to severely clotted | Reject |
| Paraffin embedded bone marrow aspirate clot or biopsy blocks | Reject |
Day(s) Performed
Monday through Friday