Culture Acid Fast Bacili
Icon & Photos
Sterile Container
Specimen Requirements
Specimen:
Sputum: should all be first morning collections.
Urine: should all be first morning collections.
Tissue: collected in a Sterile Container
Body Fluid: collected in a Sterile Container
Minimum Volume: 1.0 mL
Collection Instructions: Collect specimen aseptically, minimizing contamination with indigenous microbiota.
Collect sufficient material for the tests requested. Use of an insufficient quantity of specimen may result in false-negative results.
DO NOT use any fixatives or preservatives.
Swabs are NOT ACCEPTED for the isolation of mycobacteria.
Specimen Stability
Ambient: Send to Lab as soon as possible.
Refrigerate: 2 Weeks
Reference Range
No AFB Seen
No Growth in 8 Weeks
Methodology
manual
CPT
87116
Test Alias
Culture Acid Fast Bacili
Acid Fast Bacili Culture
AFB Culture
C AFB
Acid Fast Culture and Smear
Lab Handling
Use a sterile, leakproof container. Properly label the container with patient’s information: name, date of birth, SSN or other patient identificaion, date and time of collection, source of specimen. All specimens must be collected in a sterile manner. Sputum specimens should be collected as a first morning specimen (these give the best results) and should not exceed 15 ML. Sputum expectation should be from deep in the chest. Nasopharyngeal secreations or saliva should be avoided as well as the use of mouthwash before obtaining the specimen. The pooling of specimens is not acceptable as a reduced number of positive specimens may result. Specimens ordered times three should be collected three mornings in a row. Urine specimens should be collected in a sterile container and as clean-catch or catheterized specimens. These should be collected as first morning specimens. A minimum of three first morning specimens should be collected. Skin scrapings and aspirations from skin submitted for culture of Mycobacterium lepra or Mycobacterium ulcerans should be labelled as such.
NO Preservatives